Effect of PEA- LUT combination therapy on paw edema and body weight. CIA developed rapidly in mice immunized with CII, leading to a 100% incidence of CIA at day 28 (B). Swelling of hindpaws (A) over time was measured at 2-day intervals. Beginning on day 25, the CII-challenged mice gained significantly less weight, and this trend continued through day 35 (C). CIA-PEA mice demonstrated a significant reduced incidence of weight loss (C), as well as less paw edema (A). CIA-LUT mice did not demonstrate a reduced incidence of weight loss (C) as well as less paw edema (A) compared with the PEA group. Furthermore, the combination therapy with PEA and LUT enhanced the reduction of incidence of body-weight loss and paw edema (A, C). Figure is representative of all the animals in each group. Values are expressed as mean ± SEM of 20 animals for each group. *P < 0.01 versus Sham-control. °P < 0.01 versus CIA. #
P < 0.01 versus CIA-PEA. §
P < 0.01 versus CIA-LUT.