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Figure 6 | Arthritis Research & Therapy

Figure 6

From: NFAT3 and TGF-β/SMAD3 regulate the expression of miR-140 in osteoarthritis

Figure 6

ChIP assays of NFAT3 and SMAD3 on rsmiR-140.. Human osteoarthritic chondrocytes (n = 7 to 8) in A) NFAT3 chromatin immunoprecipitation (ChIP) assay and representative gels using primers specific to the B) rsmiR-140 sequence (amplified fragment of 253 bp) and C) unrelated control gene MAP1A (amplified fragment of 211 bp); D) SMAD3 ChIP assay and representative gels using primers specific to the E) rsmiR-140 sequence (amplified fragment of 253 bp) and F) unrelated control gene MAP1A (amplified fragment of 211 bp). IgG: pre-immune IgG, input: 1% of the starting genomic DNA lysate. Cells received no treatment (control) or were treated with B), C) ionomycin or E), F) TGF-β. Each control (panels A and D) was assigned an arbitrary value of 1 and the amplification evaluated as fold change over control. P values were assessed by the one-sample t-test, comparing treated chondrocytes to controls. IgG, immunoglobulin G; TGF-β, transforming growth factor β.

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