Immune response profiling in early rheumatoid arthritis: discovery of a novel interaction of treatment response with viral immunity

Table 3 Principal components analysis identifies immune response profiles defined by multiplexed detection of ex vivo cytokine production in 71 patients with early RA

Cytokine	CD3/CD28		CMV/EBV		CpG		PHA		PMA		SEA/SEB		Media
G-CSF	-0.158	0.112	-0.224	0.540	0.736	-0.095	-	0.933	0.802	-0.131	0.883	0.085	0.334	0.564
GM-CSF	0.202	0.795	0.186	0.828	0.438	-0.005	0.300	0.225	0.700	0.343	0.706	0.388	0.829	0.111
IFN-γ	0.709	0.101	0.690	0.176	0.495	0.013	0.708	0.523	0.144	0.283	0.061	0.613	0.196	0.623
IL-1β	0.178	0.202	0.086	0.306	0.722	-0.116	0.224	0.722	0.568	0.093	0.609	0.561	0.196	0.685
IL-2	0.693	0.298	0.798	0.211	-0.194	0.291	0.586	0.089	0.136	0.418	0.243	0.422	0.167	-0.055
IL-4	0.783	0.103	0.898	0.152	-0.055	0.932	0.892	0.165	-0.047	0.647	0.020	-0.040	-0.069	-0.131
IL-5	0.189	0.184	0.858	0.104	-0.083	0.739	0.825	0.260	-0.005	0.207	0.095	0.217	-0.138	-0.029
IL-6	0.101	0.716	-0.320	0.495	0.632	-0.107	0.298	0.820	0.772	0.012	0.827	0.319	0.642	0.532
IL-7	0.154	0.181	0.013	-0.102	-0.018	0.051	0.141	0.343	0.349	0.076	0.030	0.055	0.133	0.030
IL-8	0.177	-0.502	-0.117	-0.386	-0.727	0.146	-0.233	-0.748	-0.925	0.088	-0.480	-0.656	-0.151	-0.394
IL-10	0.830	0.152	0.328	-0.142	0.085	0.075	0.790	0.320	-0.006	0.469	0.145	0.060	0.121	0.130
IL-12	0.755	-0.174	0.787	0.110	-0.216	0.880	0.686	0.009	-0.058	0.800	-0.051	-0.004	-0.098	-0.128
IL-13	0.377	0.254	0.908	0.159	0.104	0.413	0.777	0.375	0.316	0.209	0.170	0.038	0.036	0.025
IL-17	0.157	0.786	0.369	0.787	0.024	0.073	0.247	0.054	0.283	0.207	0.841	0.175	0.612	0.054
MCP-1	-0.703	0.311	0.305	0.759	0.510	-0.109	0.252	0.650	0.682	-0.067	0.798	0.047	0.775	0.253
MIP-1β	0.484	0.247	0.100	0.444	0.543	-0.010	0.681	0.519	0.147	0.660	0.362	0.376	0.591	0.357
TNF-α	0.765	0.424	0.268	0.159	0.805	0.018	0.661	0.584	0.768	0.448	0.228	0.750	0.600	0.539

Shown are the loadings for each cytokine in the first (left column for each stimulus) and the second (right column for each stimulus) principal components. CMV, cytomegalovirus; EBV, Epstein-Barr virus; CpG, CpG oligonucleotides; PHA, phytohemagglutinin; PMA, phorbol myristate acetate with ionomycin; SEA/SEB, staphylococcal enterotoxins A and B; G-CSF, granulocyte colony stimulating factor; GM-CSF, granulocyte monocyte colony stimulating factor; IFN, interferon; IL, interleukin; MCP-1, monocyte chemoattractant protein 1; MIP-1β, monocyte inflammatory protein 1-beta; TNF-α, tumor necrosis factor-α.

ISSN: 1478-6362