Figure 5

Gene expression of FMS-related tyrosine kinase 3 ligand, CD135 and tumor necrosis factor-converting enzyme by in vitro polarized human macrophages and monocyte-derived dendritic cells. Macrophages and monocyte-derived dendritic cells (mo-DCs) differentiated as stated in Methods. (A) Interferon-gamma (IFNγ)-differentiated macrophages expressed higher levels of FMS-related tyrosine kinase 3 ligand (Flt3L) compared with all the other polarizing conditions (IFNγ vs. monocytes, P = 0.0317; IFNγ vs. interleukin (IL)-10, P = 0.0079; IFNγ vs. macrophage colony-stimulating factor (M-CSF) and granulocyte–macrophage colony-stimulating factor (GM-CSF), P = 0.0043). There was a trend for higher Flt3L expression in mo-DC compared with monocytes but these differences did not reach statistical significance. (B) CD135 expression is higher in monocytes and is downregulated upon differentiation into macrophages. There was a trend for lower of CD135 expression in mo-DC compared with monocytes but these differences were not statistically significant. (C) Tumor necrosis factor-converting enzyme (TACE) expression was similar in monocytes and polarized macrophages and in monocytes and mo-DC. Bars represent mean ± standard error of the mean of at least five independent donors’ mRNA expression of each gene relative to Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). *P <0.05, **P <0.01, ***P <0.001. monos.