Biochemical composition of neocartilage normalized to tissue wet weight and DNA. (A) Chondroitinase ABC (C-ABC) and transforming growth factor beta-1 (TGF-β1) individually enhanced collagen density (per wet weight (ww)), and C-ABC/TGF-β1 and hydrostatic pressure (HP)/C-ABC/TGF-β1 treatments synergistically enhanced collagen density (per ww). (B) Reflecting phenotypic changes, TGF-β1 led to the greatest increase in collagen production per cell. (C), (D) TGF-β1 additionally enhanced the glycosaminoglycan (GAG) content, normalized to ww and DNA, while C-ABC and combined treatments containing C-ABC showed the lowest GAG/DNA. Data reported as mean ± standard deviation. All groups not connected by a common lowercase letter are significantly different (P <0.05).