Figure 4From: Carprofen inhibits the release of matrix metalloproteinases 1, 3, and 13 in the secretome of an explant model of articular cartilage stimulated with interleukin 1βPercentage GAG release throughout the time course with IL-1β stimulation and carprofen treatment. The following treatments were applied: untreated control, IL-1β (10 ng/ml), carprofen (100 μg/ml) + IL-1β (10 ng/ml), or carprofen (100 μg/ml). The percentage GAG release was measured after 0 to 6 days and 6 to 12 days of incubation. The day 6 GAG-release data confirmed that carprofen slows IL-1β-stimulated GAG release. The GAG-release measurements for the day 12 time point would have been influenced by GAG loss during the earlier culture period. Carprofen + IL-1β samples showed higher GAG release at day 12 than did IL-1β alone, because of the high loses that had already occurred during days 0 to 6. Explant culture time courses were completed by using tissues from three separate animals, with three treatment replicates for each experiment. **P < 0. 01; ***P < 0. 001. Error bars indicate standard deviation.Back to article page