Table 1 Summary of proteins identified in untreated control and IL-1β-stimulated samples with qualitative nanoLC-MS/MS
Protein | Accession number | Mascot scores | |||
|---|---|---|---|---|---|
Individual animal Untreated control | Pooled untreated control | Individual animal IL-1β stimulated | Pooled IL-1β stimulated | ||
ECM proteins | |||||
Cartilage oligomeric matrix protein | COMP_BOVIN | 4090 | 4763 | 7555 | 10590 |
Fibronectin | FINC_BOVIN | 4422 | 6222 | 3786 | 3492 |
Chondroadherin | CHAD_HUMAN | 1022 | 3069 | 1978 | 3689 |
Thrombospondin 1 | TSP1_BOVIN | 741 | 1256 | 3456 | 2782 |
Aggrecan core protein | PGCA_HUMAN | 1815 | 3389 | 2258 | 6412 |
Decorin | PGS2_HORSE | 665 | 802 | 513 | 1130 |
Biglycan | PGS1_HORSE | 1099 | 1062 | 2047 | 1720 |
Fibromodulin | FMOD_HUMAN | 1307 | 3200 | 1381 | 1930 |
Collagen alpha-1(II) chain | CO2A1_BOVIN | 137 | 59 | 133 | 82 |
Collagen alpha-2(VI) chain | CO6A2_HUMAN | 190 | 79 | ||
Collagen alpha-1(X) chain | COAA1_MOUSE | 77 | 102 | ||
Prolargin | PRELP_MOUSE | 124 | 147 | 311 | 301 |
Cartilage intermediate-layer protein 1 (CILP-1) | CILP1_MOUSE | 201 | 789 | 423 | 264 |
Proteoglycan 4 (Lubricin) | PRG4_HUMAN | 139 | 49 | 59 | |
SPARC (secreted protein acidic, cysteine rich) | SPRC_PIG | 108 | 96 | ||
Non-ECM secreted proteins | |||||
Clusterin | CLUS_HORSE | 2767 | 4723 | 1785 | 4876 |
Chitinase-3-like protein 1 (YKL-40) | CH3L1_BOVIN | 574 | 1178 | 414 | 2018 |
Serum amyloid A protein | SAA_CANFA | 43 | |||
SAA_HORSE | 55 | 219 | 436 | ||
Metalloproteinase inhibitor 1 | TIMP1_HORSE | 83 | 312 | 457 | 146 |
Alpha-1-antiproteinase 2 | A1AT2_HORSE | 99 | 342 | 50 | |
MMP-1 | MMP1_HORSE | 1678 | 1586 | ||
MMP-3 | MMP3_HORSE | 653 | 956 | 5441 | 4580 |
MMP-13 | MMP13_HORSE | 1700 | 699 | ||
TNF-R superfamily, member 11b (Osteoprotegerin) | TR11B_RAT | 252 | 319 | 388 | 638 |
Procollagen C-endopeptidase enhancer 2 | PCOC2_HUMAN | 163 | 171 | ||
Lysozyme C | LYSM_BOVIN | 72 | 168 | 179 | 148 |
Secreted frizzled-related protein 3 | SFRP3_BOVIN | 74 | 98 | ||
Lactadherin | MFGM_PIG | 83 | 63 | 54 | |
Extracellular superoxide dismutase [Cu-Zn] | SODE_HUMAN | 67 | 72 | ||
Macrophage migration inhibitory factor | MIF_HUMAN | 91 | 86 | ||
Ribonuclease 4 | RNAS4_HUMAN | 48 | 46 | 59 | |
Intracellular proteins | |||||
Vimentin | VIME_PANTR | 84 | |||
VIME_PIG | 2361 | 3837 | |||
Alpha-enolase | ENOA_BOVIN | 506 | 686 | ||
Phosphoglycerate mutase 1 | PGAM1_MOUSE | 149 | 104 | ||
Phosphatidylethanolamine-binding protein 1 | PEBP1_HUMAN | 423 | 148 | ||
Phosphoglycerate kinase 1 | PGK1_HORSE | 792 | 651 | ||
Pyruvate kinase isoforms M1/M2 | KPYM_HUMAN | 488 | 245 | ||
Purine nucleoside phosphorylase | PNPH_HUMAN | 175 | 134 | ||
Glyceraldehyde 3-phosphate dehydrogenase | G3P_PIG | 208 | 115 | ||
Thioredoxin | THIO_HORSE | 145 | 143 | ||
Other | |||||
Serum albumin | ALBU_BOVIN | 283 | 86 | ||
Pancreatic trypsin inhibitor | BPT1_BOVIN | 283 | 477 | 281 | 703 |
Trypsin | TRYP_PIG | 111 | 106 | 746 | |
Keratin, type II cytoskeletal 3 | K2C3_RABIT | 151 | 277 | 110 | |