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Table 2 Cytokine responses from mice treated with oral A12

From: Characterization of T cell phenotype and function in a double transgenic (collagen-specific TCR/HLA-DR1) humanized model of arthritis

Treatment Culture Cytokines (pg/ml)
   IFN-γ IL-17 IL-10 IL-4
PBS No Ag 10 ± 7 17 ± 8 17 ± 10 5 ± 3
  mA2 280 ± 22 256 ± 28 45 ± 14 25 ± 6
A12 No Ag 2 ± 3 2 ± 1 15 ± 9 4 ± 3
  mA2 12 ± 20* 2 ± 2* 105 ± 18* 48 ± 8*
  1. Pooled cells from Peyer’s patches and mesenteric lymph nodes from A12-treated DR1/T cell receptor (TCR) Tg mice were taken from the mice 10 days after treatment with oral gavage was completed, and were cultured with murine A2 peptide (mA2) (3 μmol/ml) so that supernatants could be analyzed for the presence of cytokines. When cultures from mice treated with A12 were compared with those from mice treated with PBS alone, the IL-10 response to mA2 was elevated whereas the inflammatory cytokines (IFN-γ and IL-17), were suppressed. The numbers indicated represent the means ± standard error of the mean of three separate experiments. *P ≤0.01 when type II collagen (CII) responses from A12-treated mice were compared with CII responses from untreated mice.