Treatment | Culture | Cytokines (pg/ml) |
|---|
| | | IFN-γ | IL-17 | IL-10 | IL-4 |
|---|
PBS | No Ag | 10 ± 7 | 17 ± 8 | 17 ± 10 | 5 ± 3 |
| | mA2 | 280 ± 22 | 256 ± 28 | 45 ± 14 | 25 ± 6 |
A12 | No Ag | 2 ± 3 | 2 ± 1 | 15 ± 9 | 4 ± 3 |
| | mA2 | 12 ± 20* | 2 ± 2* | 105 ± 18* | 48 ± 8* |
- Pooled cells from Peyer’s patches and mesenteric lymph nodes from A12-treated DR1/T cell receptor (TCR) Tg mice were taken from the mice 10 days after treatment with oral gavage was completed, and were cultured with murine A2 peptide (mA2) (3 μmol/ml) so that supernatants could be analyzed for the presence of cytokines. When cultures from mice treated with A12 were compared with those from mice treated with PBS alone, the IL-10 response to mA2 was elevated whereas the inflammatory cytokines (IFN-γ and IL-17), were suppressed. The numbers indicated represent the means ± standard error of the mean of three separate experiments. *P ≤0.01 when type II collagen (CII) responses from A12-treated mice were compared with CII responses from untreated mice.
