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Table 2 Mass spectrometry and DIGE quantification data of synovial membrane proteins

From: Synovial membrane protein expression differs between juvenile idiopathic arthritis subtypes in early disease

Spot number

Protein

Gene symbol

Accession #

Mowse score

% Sequence coverage

CalculatedpI value

Nominal mass (Mr)

Number matched peptides

Normalised volume (Oligo)

Normalised volume (Poly)

ANOVAP-value

1

No ID

-

-

-

-

-

-

-

0.73

3.02

0.001

2

Protein kinase C inhibitor protein 1

KCIP-1

gi|114629705

88

40

4.78

24,166

14

1.07

2.1

0.03

 

Annexin

A5 ANXA5

gi|149698420

79

33

4.98

35,977

10

-

-

-

3

Myosin regulatory l ight chain 9

MYL9

gi|29568111

52

29

4.8

19,814

8

0.92

2.02

0.04

4

No ID

-

-

-

-

-

-

-

0.79

2.35

0.006

5

Beta Actin

ACTB

gi|15277503

76

21

5.55

40,194

9

1.14

2.28

0.04

6

Beta Actin

ACTB

gi|15277503

176

37

5.55

40,194

17

0.35

1.24

0.02

7

Fibrinogen gamma chain

FGG

gi|930064

94

50

6.49

24,109

11

0.88

2.23

0.004

8

Beta actin variant

ACTB

gi|62897625

451

53

5.37

41,738

23

0.56

1.94

0.005

9

No ID

-

-

-

-

-

-

-

0.64

2.04

0.02

10

Chain A, profi l in-beta-actin

PFN1

gi|157111829

106/130

44

5.29

41,616

21

0.39

1.76

0.04

11

F-actin capping protein alpha-1

CAPZA1

gi|5453597

196

55

5.45

32,902

17

0.3

1.38

0.03

12

Myosin regulatory l ight chain 2

MYL2

gi|222144328

86

55

4.26

17,745

10

0.66

2.03

0.02

13

No ID

-

-

-

-

-

-

-

0.62

1.72

0.05

14

Peroxiredoxin

2 PRDX2

gi|4758638

348

57

6

25,019

18

0.3

1.73

0.02

15

Chain C, fibrinogen fragment D

FFD

gi|2781209

89

42

5.87

36,157

12

2.08

0.58

0.0002

16

Fibrinogen gamma chain

FGG

gi|930064

71

44

6.49

24,109

7

0.9

2.14

0.05

17

Collagen, type VI, alpha 2

COL6A2

gi|41350923

81

18

5.85

182,860

18

2.77

1.09

0.03

18

Chain C, fibrinogen fragment

D FFD

gi|24987625

102

44

5.86

35,155

14

2.1

0.96

0.005

19

No ID

-

-

-

-

-

-

-

1.6

0.9

0.008

20

No ID

-

-

-

-

-

-

-

1.6

0.8

0.02

21

No ID

-

-

-

-

-

-

-

1.4

0.7

0.006

22

No ID

-

-

-

-

-

-

-

   

23

Integrin, alpha 2b

ITGA2B

gi|119571979

95

15

5.41

103,127

16

1.87

0.64

0.04

24

Alpha 3 type VI collagen

COL6A3

gi|62088852

119

17

8.73

182,860

27

1.78

0.78

0.05

25

No ID

-

-

-

-

-

-

-

1.72

0.74

0.04

26

No ID

-

-

-

-

-

-

-

1.56

0.72

0.02

27

Peroxiredoxin 6

PRDX6

gi|4758638

348

57

6

25,019

18

-

-

-

28

Triosephosphate isomerase 1

TPI1

gi|17389815

111

53

6.45

26,625

15

-

-

-

29

Albumin, isoform CRA_j

ALB

gi|119626073

59

23

6.4

26,233

7

-

-

-

30

Calreticul in precursor variant

CRT

gi|62897681

236

29

4.3

46,890

19

-

-

-

31

Fibrinogen, beta chain

FGB

gi|70906435

93

34

8.54

55,892

21

-

-

-

  1. Spot trypsin digests were identified using matrix assisted laser desorption ionization (MALDI-TOF/TOF), correlated to compiled peptide data (Matrix Science). P-values highlight inter-subgroup comparisons which reached statistical significance (P <0.05) by ANOVA. Full mass spectra peak lists for each identified protein are provided in Additional file1: Table S1.