Figure 4

miR-26a and toll-like receptor (TLR)3 expression in rat macrophages after pristane stimulation in vitro . (A) RT-qPCR results of tlr3 mRNA and miR-26a expression and (B) western blotting results of TLR3 protein expression in NR8383 after stimulated by 1 mM pristane emulsion for 24 h. (C) RT-qPCR results of miR-26a, tlr3, ifn-β and tnf-α mRNA expression, (D) western blotting results of TLR3 protein expression in NR8383 and (E) ELISA results of TNF-α protein expression in the cell supernatant after incubation with 10 nM mimics and inhibitors for 24 h and 1 mM pristane stimulation for another 24 h. U6 snRNA and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as internal controls in RT-qPCR for miRNA and mRNA expression detection, respectively. Bars represent the standard error of the mean from three experiments. *Statistically significant differences compared with medium control in (A) and (B). In (C) and (D), *significant differences compared with the negative control miRNA group; ^significant differences compared with the mock group (Mann–Whitney U-test, P <0.05). One representative plot and quantitative data from three independent western blotting tests are shown. Ratio indicates the optical intensity of TLR3 protein bands against GAPDH.