Figure 6
Evaluation of miR-26a mimic treatment in pristine-induced arthritis (PIA) rats. (A) A brief scheme for miR-26a mimic treatment in PIA rats. D, day. (B) Arthritis clinical score curve from the onset day to D23. (C) Mean ankle perimeter and (D) mean foot-pad perimeter of rats. (E) Quantative data for pathological scores indicating synovitis of arthritis (left panel) and representative H&E staining of rat ankle from each group (right panel), scale:1.0 mm. (F) miR-26a expression detected by stem loop qRT-PCR in spleens of miR-26a-treated rat spleens. U6 snRNA was chosen as THE endogenous control. (G) TLR3 protein expression detected by western blotting. Upper bands show representative plots of toll-like receptor (TLR)3 and endogenous glyceraldehyde-3-phosphate dehydrogenase (GAPDH) control using protein samples from rat spleen. Lower figure shows the quantitative data for the protein expression ratio calculated using rat spleen protein samples from all groups. (H) TNF-α protein expression detected by ELISA in rat plasma from all groups. Error bar represents mean ± standard error of the mean of each group (n = 8). *Statistically significant differences compared with PIA + saline control; ^significant differences compared with PIA + negative control (NC): *,^P <0.05; **P <0.01 (Mann–Whitney U-test).