Figure 5

Indian hedgehog, matrix metalloprotease 13 and type X collagen expression is reduced in Ihh-deleted mice, but type II collagen and aggrecan are preserved. Immunohistochemical staining was used in osteoarthritis (OA)-induced tibial articular cartilage of 5-month-old mice for Indian hedgehog (Ihh) (A), matrix metalloprotease 13 (MMP-13) (B), type X collagen (Col X) (C), type II collagen (Col II) (D) and type II collagen breakdown products (Degraded Col II) (E). PMM, partial medial meniscectomy; TM, tamoxifen. Positive signals (brown staining) are indicated by arrows. Inside the black box was image taken under the 20x objective lens, and from which a small area was further enlarged under 20x objective lens (inside the red box)