Figure 2
From: Methotrexate induces production of IL-1 and IL-6 in the monocytic cell line U937
Production of IL-1 and IL-6 measured by ELISA in supernatants of U937 cells after 72 hours of culture with MTX showed dose-dependent increases (top panels). Similar increases in corresponding gene expression levels for IL-1 and Il-6, expressed as Delta Ct values, also were measured (bottom panels). P values were determined by one-way ANOVA and post hoc analyses confirmed significant (P <0.05) increases at MTX 1.0 uM (indicated by asterisks) compared to cultures with no added MTX for all four measures. ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; MTX, methotrexate.