Figure 4

Fucosyltransferase 1 (Fut1) siRNA inhibited pro-angiogenic mediator production in TNF-α-stimulated rheumatoid arthritis (RA) synovial fibroblasts. (A) Monocyte chemoattract protein 1 (MCP-1/CCL2) mRNA expression in fut1 siRNA-treated RA synovial fibroblasts was 60 ± 9% (mean ± standard error (SE)) of control siRNA-transfected RA synovial fibroblasts, showing a 40% reduction in MCP-1/CCL2 mRNA expression in fut1 siRNA-transfected cells. (B) Epithelial-derived neutrophil-activating peptide 78 (ENA-78/CXCL5) mRNA expression in fut1 siRNA-treated RA synovial fibroblasts was 69 ± 11% (mean ± SE) of control siRNA transfected RA synovial fibroblasts, showing a 31% reduction in ENA-78/CXCL5 mRNA expression fut1 siRNA-transfected cells. (C) Vascular endothelial growth factor (VEGF) mRNA expression in fut1 siRNA-treated RA synovial fibroblasts was 79 ± 7% (mean ± SE) of control siRNA-transfected RA synovial fibroblasts, showing a 21% reduction in VEGF mRNA expression fut1 siRNA-transfected cells. (D) Fut1 siRNA inhibited production of MCP-1/CCL2. (E) Fut1 siRNA inhibited production of ENA-78/CXCL5. (F) Fut1 siRNA inhibited production of VEGF. Means are presented with SE. *P <0.05 was significant (n = number of RA patient synovial fibroblasts).