Diminished endothelial repair by treatment with anti-AT
R and anti-ET
R Ab-positive SSc-IgG . HMEC-1 treated with anti-AT1R and anti-ETAR Ab-positive SSc-IgG reduce wound areas measured by a scratch assay. (A) Representative pictures of NC-IgG and of SSc-IgG-treated cells and indicated inhibitors. (B) After 24 hours, wound areas are significantly greater in SSc-IgG versus NC-IgG treatment (NC-IgG, n = 6, SSc-IgG n = 6, P < 0.01) and are significantly reduced by antagonists, as indicated (P < 0.05). Bar indicates 250 micron. Independent experiments were performed at least twice. Mean and SEM, **P < 0.01 and *P < 0.05. (C) Wound area, as measurement of endothelial repair, shows a correlation to levels of anti-AT1R Abs and anti-ETAR Abs (NC-IgG, n = 11; SSc-IgG, n = 14; total n = 25). Spearman correlation.