Time point and B-cell subset distribution of the large expanded clone. The graphical ClustalX alignment of all of the expanded clone members from Sjögren’s syndrome subject 2 (SjS2) is based upon their degree of nucleotide sequence similarity. The circles at the end of each branch indicate the time point, and the colors of the branches show the memory B-cell subset from which the clone member was recovered. Blue indicates CD38++ (plasmablasts, PB), and black indicates CD38− (memory cells). GL, inferred germline sequence.