Angiotensin II receptor type 1 and endothelin receptor type A are expressed in human peripheral blood mononuclear cells, their protein expression is decreased in systemic sclerosis patients. Protein expression of both receptors in CD3+ T cells, CD19+ B cells and CD14+ monocytes of systemic sclerosis (SSc) patients (n = 18) and healthy donors (n = 14) was measured by flow cytometry. (A) Density of angiotensin II receptor type 1 (AT1R) and endothelin receptor type A (ETAR) (B) is represented by the median fluorescence intensity (MFI) normalized to the isotype control. (C) Frequency of AT1R-positive cells and (D) ETAR-positive cells is represented by the percentage relative to an isotype control. (E) AT1R mRNA (AGTR1) expression and (F) ETAR mRNA (ENDRA) expression in CD3+ T cells, CD19+ B cells and CD14+ monocytes of healthy donors (n = 3) was measured by real-time PCR and calculated using the 2-ΔCt method. Results are represented relative to the housekeeping gene elongation factor 1 α1 (EF1A1). Statistical analysis was carried out by Mann–Whitney U test. Data are shown as box-and-whisker plots (Tukey). *P < 0.05 and **P < 0.01.