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Figure 6 | Arthritis Research & Therapy

Figure 6

From: Growth differentiation factor 6 and transforming growth factor-beta differentially mediate mesenchymal stem cell differentiation, composition, and micromechanical properties of nucleus pulposus constructs

Figure 6

Analysis of BM and AD-MSC response to growth-factor stimulation in pellet culture. Quantitative real-time PCR analysis of gene-expression changes in (A), BM-MSCs and (B), AD-MSCs in pellets in the absence or presence of TGF-β, GDF5, or GDF6. Relative gene expression was normalized to mean housekeeping-gene expression and cells without growth-factor stimulation and plotted on a log scale. (C) Aggrecan-to-type II collagen gene-expression ratio in BM and AD-MSCs after culture in type I collagen hydrogels for 14 days with either no growth factor, TGF-β, GDF5, or GDF6. Relative gene expression was normalized to mean housekeeping-gene expression and plotted on a log scale. (D) DMMB quantification of sGAG content within pellets and cumulative release into media over 14 days normalized to DNA content of the pellet at day 14. For all analyses, N = 3; data represent mean ± SEM. *P < 0.05.

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