Figure 4

Decreased numbers of CD8⁺T cells and macrophages at the sites of C protein-induced myositis (CIM) in mast cell-deficient W/W^vmice. CIM was induced in W/W^v mice and control congenic wild-type (WT) mice. Twenty-one days after the induction, specimens of muscle were stained with anti-CD8 antibody, anti-F4/80 antibody, and TO-PRO-3 (nuclear staining). Representative photomicrographs of CD8⁺ cells (a) and F4/80⁺ cells (b) in skeletal muscle in W/W^v mice and WT mice are shown. Mean ± standard deviation of the numbers of CD8⁺ cells (c) and F4/80⁺ cells (d) (per mm²) is shown. n = 6 mice in each group. *Significantly different from the mean value of WT mice (P <0.01). ND, not detectable.