Levels of C-protein-specific immunoglobulins in sera. Sera were obtained from wild-type (WT) mice before (as a control) and at 21 days after C protein-induced myositis (CIM) induction, diluted to indicated rates, and subjected to enzyme-linked immunosorbent assay (ELISA) for anti-C protein IgG (a) or anti-C protein IgE (b). The optical density (OD) levels of anti-C protein IgE in CIM-induced mice were nearly identical to those in control mice. (c) Sera obtained from WT mice and W/Wv mice at 21 days after CIM induction were diluted by a 100-fold and subjected to ELISA for IgG subclasses against C protein. Data are mean ± standard deviation; n = 5, each.