Vascular leakage in skeletal muscle in wild-type (WT) mice and W/Wvmice upon C protein-induced myositis (CIM) induction. CIM was induced in WT mice and W/Wv mice, and at 10 and 21 days after the induction, Evans blue leakage in skeletal muscle was evaluated as described in Materials and methods. (a) Representative photomicrographs of phase contrast, Evans blue leakage, and merged image are shown. Arrowheads indicate red fluorescence by Evans blue dye leakage in extravascular space in the perimysium. (b) Mean ± standard deviation of the mean fluorescence intensity (MFI) of Evans blue dye in the perimysium is shown. n = 6 mice in each group. *Significantly different from the mean value of WT mice (P <0.01).