Skip to main content
Figure 2 | Arthritis Research & Therapy

Figure 2

From: Accelerated and increased joint damage in young mice with global inactivation of mitogen-inducible gene 6 after ligament and meniscus injury

Figure 2

Cartilage and subchondral bone cell analysis. (A) Ki67 stain (brown) of knee tissue from wild-type (WT) and inactivated mitogen-inducible gene 6 (Mig-6−/−) mice at 4 weeks after surgery. Arrows point to stained cells and cartilage (c) and subchondral bone (b) are labeled. For WT mice, osteoblasts are stained in the subchondral bone and chondrocytes are stained in the cartilage. For Mig-6−/−mice, osteoblasts are stained in the subchondral bone, chondrocytes are stained in the control knee cartilage and both fibroblasts and chondrocytes are stained in the surgically treated knee cartilage. (B) Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) stain of WT and Mig-6−/−tissues at 4 weeks postsurgery. Apoptotic cells are stained green, and nuclei are stained blue. c = cartilage and b = subchondral bone. For WT mice, osteoblasts are stained in the subchondral bone and chondrocytes are stained in the cartilage. For Mig-6−/−mice, osteoblasts are stained in the subchondral bone, chondrocytes are stained in the control knee cartilage and both fibroblasts and chondrocytes are stained in the surgically treated knee cartilage. ImageJ software was used to quantify the area of positive TUNEL staining (mean ± SE). (C) RT-PCR data for WT and Mig-6−/−surgically treated knees (relative to control knees) at 4 weeks after surgery, expressed as mean ± SE. **P < 0.01 between genotypes and *P < 0.05 between genotypes. The amplification of gene products was determined by the incorporation of SYBR Green I Nucleic Acid Gel Stain (Life Technologies, Carlsbad, CA, USA) and normalized to 18 s using the 2ΔΔ−Ct method. Col10a1: Collagen, type X, α1; Col1a1: Collagen, type I, α1; Col2a1: Collagen, type II, α1; Ocn-1: Osteocalcin variant 1; Ocn-2: Osteocalcin variant 2; Opn: Osteopontin; Osx: Osterix; Runx2: Runt-related transcription factor 2.

Back to article page