Figure 4

Mice used for micro-CT analysis were also used for enzyme-linked immunosorbent assay measurements. (A) Serum receptor activator of nuclear factor κB ligand (RANKL) concentration for wild-type (WT) and inactivated mitogen-inducible gene 6 (Mig-6^−/−) mice expressed as mean ± SE. *P < 0.05. (B) Serum osteoprotegerin (OPG) concentration for WT and Mig-6^−/−mice expressed as mean ± SE. ***P < 0.001 and ****P < 0.0001. (C) Arrows point to tartrate-resistant acid phosphatase (TRAP)-positive cells (pink) in the subchondral bone of knees from WT and Mig-6^−/−mice 2 weeks after surgery. The number of TRAP-stained cells was counted manually and normalized to the tissue area (mean ± SE). (D) Arrows point to TRAP-positive cells (pink) in the subchondral bone of knees from WT and Mig-6^−/−mice 4 weeks after surgery. The number of TRAP-stained cells was counted manually and normalized to the tissue area (mean ± SE).