Endothelin-1 (ET-1) promoted the nuclear localization of protein kinase C ( PKC)-δ by activating c-Abl. ( A ) Normal dermal fibroblasts were treated with 200 nM of ET-1 for the indicated period of time. The protein levels of c-Abl, PKC-δ, and β-actin and the phosphorylation levels of c-Abl were determined by immunoblotting using whole cell lysates (WCL). ( B ) Normal dermal fibroblasts were treated with ET-1 for 30 minutes and cytoplasmic and nuclear extracts were prepared. The protein levels of PKC-δ in the cytoplasm and nucleus were determined by immunoblotting. Equal amounts of loading were confirmed by immunoblotting for β-actin in cytoplasmic extracts and for TATA binding protein (TBP) in nuclear extracts. The values below each blot represent the relative levels of target molecules normalized by loading controls with densitometry.