Figure 3

miR-155 promotes the production of inflammatory cytokines over monosodium urate (MSU) crystal stimulation. miR-155 or scrambled control were mixed with Lipofectamine 2000 Reagent in serum-free RPMI and transfected into the THP-1 cells. After 24 h of transfection, cells were treated with phorbol 12-myristate 13-acetate/ionomycin (PMA) (100 ng/ml) for 3 h. After stimulation with PMA, cells were washed and then stimulated with MSU (100 ug/ml) for 24 h. Human TNF-α (A) and IL-1β (B) levels in cell culture supernatants were measured using Luminex. *P <0.05, post-hoc analysis after the Kruskal-Wallis test.