Table 1 Sequences of the primers used for RT-PCR and real-time analysis a
From: Articular cartilage chondrocytes express aromatase and use enzymes involved in estrogen metabolism
Primer | Sense primers (5′ → 3′) | Antisense primers (5′ → 3′) | Base pair | Temperature |
|---|---|---|---|---|
Aromatase | CGA GAT CGA AAT TCT GGT GGA AAA G | TGC AAA ATC CAT ACA GTC TT | 177 | 60°C |
CYP1A1 | CCT CTT TGG AGC TGG GTT TG | GCT GTG GGG GAT GGT GAA | 229 | 60°C |
ERα | CAA TGA CTA TGC TTC AGG CTAC | CCA CCT TTC ATC ATT CCC AC | 198 | 60°C |
ERβ | GAG TCC CTG GTG TGA AGC AA | TGA GCA TCC CTC TTT GAA CC | 199 | 60°C |
β-actin | CAA GAG ATG GCC ACG GCT GCT | TCC TTC TGC ATC CTG TCG GCA | 275 | 60°C |