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Table 1 Sequences of the primers used for RT-PCR and real-time analysis a

From: Articular cartilage chondrocytes express aromatase and use enzymes involved in estrogen metabolism

Primer Sense primers (5′ → 3′) Antisense primers (5′ → 3′) Base pair Temperature
Aromatase CGA GAT CGA AAT TCT GGT GGA AAA G TGC AAA ATC CAT ACA GTC TT 177 60°C
CYP1A1 CCT CTT TGG AGC TGG GTT TG GCT GTG GGG GAT GGT GAA 229 60°C
ERα CAA TGA CTA TGC TTC AGG CTAC CCA CCT TTC ATC ATT CCC AC 198 60°C
ERβ GAG TCC CTG GTG TGA AGC AA TGA GCA TCC CTC TTT GAA CC 199 60°C
β-actin CAA GAG ATG GCC ACG GCT GCT TCC TTC TGC ATC CTG TCG GCA 275 60°C
  1. aCYP1A1: Cytochrome P4501A1; ER: Estrogen receptor.