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Table 2 Frequency of CD4+ T cells and CD4 + CD25 +/high CD127 low/- regulatory T cells in the peripheral blood and synovial fluid and synovial membrane joint samples in rheumatoid arthritis and osteoarthritis patients a

From: CD4+CD25+/highCD127low/- regulatory T cells are enriched in rheumatoid arthritis and osteoarthritis joints—analysis of frequency and phenotype in synovial membrane, synovial fluid and peripheral blood

  RA OA P-values
RA:OA RA RA RA OA OA OA
PB:SF PB:SM SF:SM PB:SF PB:SM SF:SM
PB Sample size (n) 18 22        
Sample volume (ml) 7 ± 0 7 ± 0        
Concentration of CD4+ T cells (cells /μl) 730 ± 210 690 ± 90 0.424 <0.0001b <0.0001b   <0.0001b <0.0001b  
(610 to 900) (540 to770)
CD4+CD25high (%total CD4+ T cells) 4.88 ± 2.04 4.4 ± 1.59 0.417 0.0072b 0.0381b   <0.0001b 0.004b  
(2 to 9.4) (1.9 to 8)
CD4+CD25+/highCD127low/- (% total CD4+ T cells) 6.7 ± 1.8 7.6 ± 1.8 0.135 0.0006b 0.0128b   0.0001b 0.0091b  
(4 to 11) (5 to 11)
CD4+CD25+/highCD127low/- (cells/μl) 48.9 ± 7.8 52.4 ± 4 0.099 <0.0001b 0.0004b   <0.0001b <0.0001b  
(30 to 99) (29 to 85)
SF Sample size (n) 17 22        
Sample volume (ml) 15.41 ± 12.65 11.21 ± 6.81        
(4.3 to 38) (2.2 to 23.6)
Concentration of CD4+ T cells (cells/μl) 70 ± 170 40 ± 90 0.479 <0.0001b   0.0001b <0.0001b   <0.0001b
(30 to 540) (10 to 480)
CD4+CD25high (% total CD4+ T cells) 9.97 ± 6.27 9.63 ± 4.12 0.7371 0.0072b   0.2449 <0.0001b   0.1086
(3.4 to 19) (3.3 to 22.6)
CD4+CD25+/highCD127low/- (% total CD4+ T cells) 11.8 ± 5.5 12.4 ± 5 0.727 0.0006b   0.1226 0.0001b   0.4549
(5 to 22) (4 to 26)
CD4+CD25+/highCD127low/- (cells/μl) 8.3 ± 9.4 5 ± 4.5 0.153 <0.0001b   <0.0001b <0.0001b   <0.0001b
(8to90) (4 to 100)
SM Sample size (n) 18 22        
Sample volume (mg) 3.45 ± 1.18 3.72 ± 1.3        
(2.2 to 5.9) (1.95 to 5.73)
Concentration of CD4+ T cells (cells/μg) 378 ± 250 240 ± 140 0.0336b   <0.0001b 0.0001b   <0.0001b <0.0001b
(10 to 610) (10 to 340)
CD4+CD25high (% total CD4+ T cells) 7.21 ± 3.9 7.5 ± 4.8 0.8354   0.0381b 0.2449   0.004b 0.1086
(2 to 18.4) (2.5 to 26)
CD4+CD25+/highCD127low/- (% total CD4+ T cells) 9.4 ± 3.8 11.2 ± 5.9 0.266   0.0128b 0.1226   0.0091b 0.4549
(5 to 17) (2.4 to 26)
CD4+CD25+/highCD127low/- (cells/μg) 35.5 ± 12 27 ± 11 0.025b   0.0004b <0.0001b   <0.0001b <0.0001b
(24 to 89) (14 to 73)
  1. aOA, Osteoarthritis; PB, Peripheral blood; RA, Rheumatoid arthritis; SF, Synovial fluid; SM, Synovial membrane. Collected sample volumes and calculated concentrations of CD4+ T cells are shown. Frequencies of CD4+CD25high and of CD4+CD25+/highCD127low/- cells are shown as the percentage of CD4+ T cells and for CD4+CD25+/highCD127low/- cells also as cell concentration (total cell counts per microlitre or per microgram) for PB, SF and SM in RA and OA patients. Values shown are mean ± SD (range) unless otherwise stated. bStatistically significant differences.