CD4 + CD25 +/high CD127 low/- regulatory T cells are enriched in rheumatoid arthritis and osteoarthritis joints—analysis of frequency and phenotype in synovial membrane, synovial fluid and peripheral blood

Babak Moradi; Philipp Schnatzer; Sébastien Hagmann; Nils Rosshirt; Tobias Gotterbarm; Jan Philippe Kretzer; Marc Thomsen; Hanns-Martin Lorenz; Felix Zeifang; Theresa Tretter

doi:10.1186/ar4545

Table 3 Phenotype characteristics of CD4 ⁺ CD25 ^+/high CD127 ^low/- T-cells from peripheral blood, synovial fluid and synovial membrane of rheumatoid arthritis and osteoarthritis patients ^a

From: CD4⁺CD25^+/highCD127^low/- regulatory T cells are enriched in rheumatoid arthritis and osteoarthritis joints—analysis of frequency and phenotype in synovial membrane, synovial fluid and peripheral blood

	RA			OA			P-values
Surface marker	PB	SF	SM	PB	SF	SM	RA	OA	PB	SM
Surface marker	PB	SF	SM	PB	SF	SM	PB:SM	PB:SM	RA:OA	RA:OA
CD45RA	22.9 ± 5	5 ± 3	2.8 ± 1	34.9 ± 13.6	6 ± 3	5.6 ± 2.5	0.0059^b	0.0006^c	0.0631	0.2377
CD45RA	21 (19.5 to 30.2)	4 (3 to 7)	2.8 (2 to 3.5)	30.8 (16.8 to 57.4)	7 (2 to 9)	6 (2.1 to 8.8)	0.0059^b	0.0006^c	0.0631	0.2377
CD45RO	83 ± 14.7	93 ± 7	94.7 ± 5.2	81.4 ± 8.5	92 ± 8	91.9 ± 7.2	0.4	0.0232^b	0.9554	0.6488
CD45RO	81 (70 to 99)	92 (87 to 99)	94.9 (89.4 to 99)	82 (60 to 95)	91 (81 to 98)	94.3 (80.9 to 99.8)		0.0232^b	0.9554	0.6488
CD69	3.1 ± 1.9	88 ± 2	84.8 ± 0.2	1.9 ± 0.8	82.3 ± 5.3	81.2 ± 4.8	< 0.0001^c	0.0029^d	0.1623	0.4746
CD69	2.1 (1.9 to 5.2)	85 (80 to 92)	84.6 (78 to 89)	1.8 (0.9 to 3.7)	84 (77.3 to 88)	82.6 (74.6 to 84.9)	< 0.0001^c	0.0029^d	0.1623	0.4746
CD62L	94.6 ± 1.8	5 ± 2	2 ± 1	94.7 ± 4.4	3 ± 1	3.1 ± 0.5	< 0.0001^c	0.0293^b	0.8881	0.2480
CD62L	94.5 (93.3 to 95.8)	3 (3 to 9)	2 (1.1 to 3.2)	95.3 (80.5 to 99.7)	3.5 (2 to 5)	3.1 (2.8 to 3.5)	< 0.0001^c	0.0293^b	0.8881	0.2480
CD152	12.6 ± 14	29.2 ± 12	36.2 ± 3.3	13 ± 6.5	31.5 ± 11	32.5 ± 8.2	0.0477^b	0.0049^d	0.7552	0.8530
CD152	5.7 (3.2 to 28.8)	33 (33 to 40)	36 (33 to 39)	16.3 (1 to 19)	30 (24 to 41)	32.5 (25 to 39.6)	0.0477^b	0.0049^d	0.7552	0.8530
CD154	13.5 ± 6.3	11 ± 5.7	15.1 ± 8.8	8.8 ± 5.4	12 ± 8	13.4 ± 8.2	0.5892	0.2863	0.2361	0.8486
CD154	13.4 (7 to 19.5)	13 (6 to 18)	15.1 (7 to 23)	8.5 (1.4 to 19)	11 (5 to 20)	10 (7.5 to 18)	0.5892	0.2863	0.2361	0.8486
CD274	23.5 ± 2.8	25 ± 7	27.6 ± 5.7	9.8 ± 10.4	25 ± 7	24.1 ± 7.9	0.3758	0.0313^b	0.0329^b	0.5541
CD274	23 (21 to 26)	32 (20 to 29)	31 (21 to 31)	6 (0.5 to 40)	26 (17 to 34)	24.1 (17 to 30.9)	0.3758	0.0313^b	0.0329^b	0.5541
CD279	3.3 ± 3.3	23.2 ± 12	22.6 ± 11	5.4 ± 6.5	9 ± 9	7.9 ± 10	0.0231^b	0.6677	0.8127	0.0239^b
CD279	3 (0.2 to 6.7)	24 (14 to 32)	22.6 (15 to 30.1)	2.2 (0 to 23.1)	8 (2 to 15)	8 (0.8 to 15)	0.0231^b	0.6677	0.8127	0.0239^b
GITR	30.3 ± 22.7	42.5 ± 7.3	45.5 ± 6.8	40.5 ± 12.8	49.2 ± 12	47.4 ± 11.9	0.329	0.173	0.636	0.8114
GITR	31.4 (7 to 52.4)	43 (39 to 51)	45.1 (38.9 to 52)	43.8 (18.4 to 59.1)	13.9 (33 to 59)	11.94 (30 to 58.7)	0.329	0.173	0.636	0.8114

^aGITR, Glucocorticoid-induced tumour necrosis factor receptor–related protein; OA, Osteoarthritis; PB, Peripheral blood; RA, Rheumatoid arthritis; SF, Synovial fluid; SM, Synovial membrane. Significant differences: ^bP < 0.05; ^cP < 0.001; ^dP < 0.01. CD4⁺CD25^+/highCD127^low/- regulatory T cells from PB, SF and SM were stained for cell surface markers as indicated in the left column. The percentage of cells positive for each surface marker is presented as mean ± SD and median and range. Data are representative of three RA patients) and five OA patients. Statistical analysis is shown for PB and SM because SF values did not differ those for SM.

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