Modulation of MoDC maturation by dendrimer ABP. A. the phenotype of immature MoDC was determined by flow cytometry. Expression of CD14, CD1a, CD11c, CD83, CD80 and CD86 was evaluated after six days of differentiation in the presence of IL-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). These results are representative of nine donors. B. Phenotype of mature MoDC was determined by flow cytometry. Markers of MoDC were evaluated after incubation for 38 hours in the presence or in the absence of dendrimer ABP and LPS + IFN-γ. The phenotype of non-manipulated, immature MoDC is shown as the control. These results are representative of nine donors. Mean Fluorescence Intensity (MFI) differences between staining and isotype control are shown. C. Variation of expression of MoDC maturation markers CD1a, CD83, CD80 and CD86. MFI was used to measure the level of expression of markers. Data are presented as the relative percentage of expression, as compared to controls obtained in the absence of stimulation (100%). Bars correspond to the 75th percentile (IQR). Data are from nine donors. *P <0.05. ABP, aza-bis-phosphonate; IFN-γ, interferon-gamma; IQR, interquartile range; LPS, lipopolysaccharides; MoDC, monocyte derived dendritic cells.