Matrix assisted laser desorption ionization mass spectrometry imaging identifies markers of ageing and osteoarthritic cartilage

Table 2 A number of ADAMTS-4 derived of biglycan and fibromodulin were identified with LC-MS/MS using MASCOT and PEAKS®

Protein	Amino acid preceeding peptide residue	Peptide sequence identified	Amino acid residue after	PEAKS derived 10lgP
Biglycan	N	C IEMGGNPLENSGFQPGAFDGLK	L	62.49
	K	NHLVEIPPNLPS^a	S	42.42
	K	DLPETLNELHLDHN	K	59.46
Fibromodulin	R	DCPQECDCPPNFPTAMY	C	53.23
	R	INEFSISSFC	T	29.37
	R	KVPDGLPSALEQLYLEHNNVY	S	29.91
	R	ELHLDHN^a	Q	28.85
	R	LSHNSLTNN	G	28.47
	R	KVPDGLPSALEQLYLEHNNVYSVPDSY	F	29.91
	K	IPPVNTNLEN	L	20.78

^adenotes a neopeptide indicating a probable cleavage site within a tryptic peptide identified in the MALDI-MSI experiment. Amino acids in bold indicate that the cleavage site is between these amino acids. ADAMTS, A distintegrin and metalloproteinase with thrombospondin motifs; LC, liquid chromatography; MS/MS, tandem mass spectrometry.

ISSN: 1478-6362