Effect of the -1830 T > C polymorphism on NR1H3 mRNA expression and B cells proliferation assay. (A) RT PCR products were separated by electrophoresis on 1% agarose gel and stained with EtBr. Gels were examined by ultraviolet illumination. (B) The mRNA levels were determined by quantitative-PCR normalized to β-actin mRNA expression. Each experiment was conducted in duplicate for each sample, and the results are expressed as mean ± SD for three independent experiments. The P value was determined by a paired t test. (C) The effect of the -1830 T > C polymorphism on the NR1H3 gene on the B cells proliferation assay. This is a colorimetric assay containing a tetrazolium compound. When the tetrazolium is reduced, it produces a colored formazan product that is soluble in cell culture medium and maximally absorbed at 490 nm. Assays were conducted in triplicate, and the experiments were repeated at least three times. Values are mean ± SD; *P <0.05. (D) Effect of the -1830 T > C polymorphism on the NR1H3 gene on B cells proliferation by stimulation with 200 ng/mL LPS, 3 μmol/L GW3965 and 5 μmol/L T0901317. Values are mean ± SD. The P value was determined by a paired t test. *P <0.05. LPS, lipopolysaccharide; SD, standard deviation.