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Table 2 Comparisons between the PhagoBurst and the DCFH-DA assay

From: Low production of reactive oxygen species in granulocytes is associated with organ damage in systemic lupus erythematosus

  Relative reactive oxygen species formation in SLE patients as% of formation in healthy controls  
Stimuli PhagoBurst DCFH-DA P-value
PMA 68 ± 7.7 76 ± 6.2 0.6783
E. coli 84 ± 13 103 ± 13 0.1775
S. aureus 92 ± 8.7 102 ± 6.3 0.2716
P. aeruginosa 88 ± 12 97 ± 14 0.5897
  1. Comparisons of the PhagoBurst assay with the dichlorodihydrofluorescein-diacetate (DCFH-DA) assay according to Perazzio et al. [23]. No significant differences between the methods were observed. Polymorphonuclear leukocytes (PMN) from systemic lupus erythematosus (SLE) patients (n = 15) and healthy controls (n = 15) were analysed in parallel with both methods using phorbol 12-myristate 13-acetate (PMA), E. coli, S. aureus or P. aeruginosa as stimuli. ROS formation was defined as geometric mean fluorescence intensity. Samples from each individual patient were divided by the mean value of the controls to gain the relative ROS formation in SLE-PMN as% of ROS produced in healthy controls. Values represent mean ± standard error of the mean. The two-sided Mann-Whitney test was used to calculate the level of significance.