Acid-sensing ion channel 3 decreases phosphorylation of extracellular signal-regulated kinases and induces synoviocyte cell death by increasing intracellular calcium

Table 1 Real-time PCR determines the gene expression of cytokines in WT FLS and ASIC3-/- FLS after incubation in pH 6.0 or 7.4 with or without IL-1β

Cytokine	Il-1b treatment	pH 7.4		pH 6.0
Cytokine	Il-1b treatment	WT	*ASIC3-/-*	WT	*ASIC3-/-*
IL-6	(-)	0.0018 ± 0.0009	0.0007 ± 0.0002	0.0037 ± 0.0028	0.001 ± 0.0004
IL-6	(+)	0.0886 ± 0.0218	0.1136 ± 0.013	0.1488 ± 0.0503	0.1829 ± 0.0411
MMP-3	(-)	0.0307 ± 0.0042	0.0180 ± 0.0060	0.0373 ± 0.0072	0.0207 ± 0.0097
MMP-3	(+)	1.7975 ± 0.7027	1.7750 ± 0.712	1.81 ± 0.7002	1.8055 ± 0.6042
MMP-13	(-)	0.0642 ± 0.0062	0.0677 ± 0.0308	0.0761 ± 0.006	0.0879 ± 0.0526
MMP-13	(+)	1.5977 ± 0.4506	1.5066 ± 0.5933	1.8362 ± 0.5362	1.5015 ± 0.5819

mRNA expression of IL-6, metalloproteinase (MMP)-3 and MMP-13 all significantly increased in wild-type (WT) fibroblast-like synoviocytes (FLS) and acid-sensing ion channel (ASIC3)-/- FLS treated with IL-1β when compared to FLS without IL-1β. There were no differences between WT FLS treated with pH 6.0 and those treated pH 7.4, and there was no difference between WT FLS and ASIC3-/- FLS. Results are presented as mean ± standard error of the mean.

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