Expression of CXCR3 in the lymph node and muscle of C-protein-induced myositis (CIM). (A) Immunohistochemistry of CIM muscle tissue and lymph node. CXCR3 was expressed in some cells of the lymph node cells (left, ×200) and inflammatory lesion in the muscle of CIM (right, ×400). (B) Immunostaining of cells invading myofiber in muscle of CIM. Differential interference contrast (DIC) showed myofiber and invasive cells in the middle of myofiber. The CD8 was co-stained with CXCR3 whereas CD4 was not (×1,000). (C) Immunostaining of inflammation in muscle of CIM showed F4/80 co-stained with CXCR3 (×1,000). (D) CXCR3-positive immune cells in CIM lymph node. CIM lymph node cells were analyzed by flow cytometry. CXCR3-positive cells were gated from lymph node cells. CXCR3-positive cells were analyzed by cell-specific markers (CD3 for T cells; B220 for B cells; F4/80 for macrophages).