Quantification of the area and number of CD68-positive macrophages on day 13 post-antigen-induced arthritis (AIA) induction. Photomicrographs of CD68-immunostained sections on day 13 post-AIA induction were scanned and the images were analyzed for the % area of CD68-positive cells (A) and their number (B) using Image J and Tissue Studio® software, respectively. Four sections were quantified and averaged per animal. Data points are mean ± standard error of the mean and n = 4 per group. *P = 0.023 (A) and 0.003 (B) compared to the untreated control group.