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Figure 3 | Arthritis Research & Therapy

Figure 3

From: The interaction between C5a and sphingosine-1-phosphate in neutrophils for antineutrophil cytoplasmic antibody mediated activation

Figure 3

Sphingosine-1-phosphate (S1P)-primed neutrophils for antineutrophil cytoplasmic antibody (ANCA)-induced respiratory burst and degranulation. (A) Neutrophil respiratory burst induced by patient-derived ANCA-positive IgG was measured by conversion of dihydrorhodamine-123 (DHR-123) to rhodamine-123 in S1P-primed cells in the presence and absence of CYM50358, VPC23019 and FTY720, respectively. (B) ANCA-induced neutrophil degranulation was determined by measuring the lactoferrin concentrations in the supernatant of neutrophil degranulation reaction. CYM50358, VPC23019 and FTY720 reduced ANCA-induced lactoferrin release.

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