Figure 5From: The interaction between C5a and sphingosine-1-phosphate in neutrophils for antineutrophil cytoplasmic antibody mediated activationSphingosine-1-phosphate (S1P) receptor antagonist inhibited supernatant (C5a-stimulated neutrophils) -primed neutrophils for antineutrophil cytoplasmic antibody (ANCA)-induced respiratory burst and degranulation. (A) Neutrophil respiratory burst induced by patient-derived MPO-ANCA-positive IgG or PR3-ANCA-positive IgG was measured by conversion of dihydrorhodamine-123 (DHR-123) to rhodamine-123 in supernatant-primed cells. Inhibition of S1P reduced supernatant (C5a-stimulated neutrophils)-primed neutrophils for ANCA-induced respiratory burst. (B) Inhibition of S1P reduced supernatant (C5a-stimulated neutrophils)-primed neutrophils for ANCA-induced lactoferrin release. Bars represent mean ± SD of repeated measurements of neutrophils from five independent experiments and donors.Back to article page