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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Type II collagen antibody response is enriched in the synovial fluid of rheumatoid joints and directed to the same major epitopes as in collagen induced arthritis in primates and mice

Figure 2

Specific antibody responses to recombinant collagen type II peptides in chronic and acute collagen-induced arthritis. (A) Chronic arthritis in male mice from B10.Q × (BALB/c × B10.Q) N2 mice (n = 10). Clinical arthritis is denoted on the right y axis: straight lines, mean arthritis score; dashed grey lines, mean + standard error the mean. Scoring of the mice was performed until day 201. The left y axis shows percent responders to the overlapping recombinant collagen type (rCII) peptides in sera from chronic collagen-induced arthritis (CIA) mice at day 35 (onset), day 80 (early-chronic disease), and day 210 (late-chronic disease). (B) rCII peptides detected in ≥50% of the mice are included. (C), (D), (E) Autoantibody recognition of rCII peptides in chronic CIA: dotted lines, cutoff calculated as the mean ± three standard deviations of naïve mice. Numbers above the bars correspond to the rCII peptides in the library followed by the epitope name in brackets for B-cell epitopes identified earlier. (F) CIA development in B10.Q male mice depicted as the mean arthritis score + standard error of the mean. (G) Sera from the acute CIA mice, collected on day 50, were also tested for specificity to the rCII peptides.

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