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Table 1 Cytokines in supernatants from spleen cell proliferation cultures

From: The role of total and cartilage-specific estrogen receptor alpha expression for the ameliorating effect of estrogen treatment on arthritis

  (Exp. 1) (Exp. 2) Total ERα-/- (Exp. 3) Col2α1-ERα-/-
(pg/mL)   WT WT KO WT KO
IL-17 P 850 ± 107 158 ± 33 97 ± 23 785 ± 158 715 ± 124
  E2 417 ± 80a 46 ± 18a 130 ± 41 391 ± 79b 335 ± 103b
IFNγ P 1,039 ± 69 8,881 ± 2,865 7,294 ± 3,192 4,465 ± 1,070 4,782 ± 760
  E2 800 ± 54b 2,813 ± 528b 4,926 ± 694 1,622 ± 360b 2,697 ± 340b
IL-2 P 1,038 ± 83 4,355 ± 581 4,098 ± 540 7,128 ± 1,598 4,405 ± 1,085
  E2 759 ± 64b 2,697 ± 663c 3,120 ± 640 1,247 ± 403b 531 ± 134b
IL-6 P 644 ± 48 596 ± 85 475 ± 127 523 ± 81 425 ± 59
  E2 469 ± 59b 383 ± 66d 447 ± 57 326 ± 68 227 ± 56b
IL-4 P 337 ± 80 114 ± 12 95 ± 9 120 ± 38 122 ± 48
  E2 279 ± 41 90 ± 9 102 ± 12 141 ± 30 102 ± 62
TNFα P 147 ± 27 95 ± 18 105 ± 15 169 ± 32 125 ± 15
  E2 190 ± 73 109 ± 31 94 ± 15 122 ± 20 88 ± 22
GM-CSF P 195 ± 22 877 ± 268 1,011 ± 202 430 ± 162 719 ± 361
  E2 158 ± 123 1,118 ± 246 826 ± 152 472 ± 189 1,069 ± 229
  1. The cytokine profile was investigated in supernatants of concanavalin A-stimulated spleen cell cultures from experiments 1 (n = 9-10), 2 (n = 12-17), and 3 (n = 8-11) (see Materials and methods). Values are given as mean ± standard error of the mean. aP <0.01, bP <0.05 versus placebo, Student t test, cP = 0.075, dP = 0.06. E2, estrogen; Exp., experiment; IFNγ, interferon gamma; IL, interleukin; KO, knockout; P, placebo; GM-CSF, granulocyte macrophage colony-stimulating factor; TNFα, tumor necrosis factor alpha; WT, wild-type.