Arthritis Research & Therapy

Table 1 Cytokines in supernatants from spleen cell proliferation cultures

From: The role of total and cartilage-specific estrogen receptor alpha expression for the ameliorating effect of estrogen treatment on arthritis

		(Exp. 1)	(Exp. 2) Total ERα^-/-		(Exp. 3) Col2α1-ERα^-/-
(pg/mL)		WT	WT	KO	WT	KO
IL-17	P	850 ± 107	158 ± 33	97 ± 23	785 ± 158	715 ± 124
	E2	417 ± 80^a	46 ± 18^a	130 ± 41	391 ± 79^b	335 ± 103^b
IFNγ	P	1,039 ± 69	8,881 ± 2,865	7,294 ± 3,192	4,465 ± 1,070	4,782 ± 760
	E2	800 ± 54^b	2,813 ± 528^b	4,926 ± 694	1,622 ± 360^b	2,697 ± 340^b
IL-2	P	1,038 ± 83	4,355 ± 581	4,098 ± 540	7,128 ± 1,598	4,405 ± 1,085
	E2	759 ± 64^b	2,697 ± 663^c	3,120 ± 640	1,247 ± 403^b	531 ± 134^b
IL-6	P	644 ± 48	596 ± 85	475 ± 127	523 ± 81	425 ± 59
	E2	469 ± 59^b	383 ± 66^d	447 ± 57	326 ± 68	227 ± 56^b
IL-4	P	337 ± 80	114 ± 12	95 ± 9	120 ± 38	122 ± 48
	E2	279 ± 41	90 ± 9	102 ± 12	141 ± 30	102 ± 62
TNFα	P	147 ± 27	95 ± 18	105 ± 15	169 ± 32	125 ± 15
	E2	190 ± 73	109 ± 31	94 ± 15	122 ± 20	88 ± 22
GM-CSF	P	195 ± 22	877 ± 268	1,011 ± 202	430 ± 162	719 ± 361
	E2	158 ± 123	1,118 ± 246	826 ± 152	472 ± 189	1,069 ± 229

The cytokine profile was investigated in supernatants of concanavalin A-stimulated spleen cell cultures from experiments 1 (n = 9-10), 2 (n = 12-17), and 3 (n = 8-11) (see Materials and methods). Values are given as mean ± standard error of the mean. ^aP <0.01, ^bP <0.05 versus placebo, Student t test, ^cP = 0.075, ^dP = 0.06. E2, estrogen; Exp., experiment; IFNγ, interferon gamma; IL, interleukin; KO, knockout; P, placebo; GM-CSF, granulocyte macrophage colony-stimulating factor; TNFα, tumor necrosis factor alpha; WT, wild-type.

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