Figure 6

Interleukin-6 (IL-6) regulates leukocyte and T-cell infiltration into lesional skin. (A, B) Reduced inflammation in mice treated with MR16-1 or immunized against the mIS200 peptide and challenged with bleomycin. Representative sections stained by hematoxylin/eosin at 400-fold magnification. (C, D) Decreased leukocyte counts in lesional skin of mice treated with MR16-1 or immunized against the mIS200 peptide. (E, F) Reduced number of CD3⁺ T cell by immunohistochemistry in lesional skin of mice treated with MR16-1 or immunized against the mIS200 peptide. Bars represent median_(Q1,Q3); in regard to leukocyte quantification, 36 mice were evaluated for passive immunization and 36 for active immunization. In regard to T-cell quantification, 15 randomly chosen mice (five per group) were evaluated for passive immunization as well as for active immunization. All results are normalized to mice injected with NaCl. Ab, antibody; KLH, keyhole limpet hemocyanin.