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Table 1 Ki values of the N-terminal domain of tissue inhibitor of metalloproteinases 1 (N-TIMP-1) and its variants

From: Engineering of tissue inhibitor of metalloproteinases mutants as potential therapeutics

Variant MMP-1 MMP-2 MMP-3
N-TIMP-1 3.0 1.1 1.9
Thr2 to serine 25 2.1 0.5
Thr2 to glycine 18 × 103 103 × 103 1.4 × 103
Thr2 to alanine 2090 307 126
Thr2 to leucine 93 1.0 3.2
Thr2 to isoleucine 262 5.6 20
Thr2 to valine 1.6 4.5 3.0
Thr2 to methionine 11 0.7 0.7
Thr2 to phenylalanine 42 17 13
Thr2 to asparagine 1970 16 44
Thr2 to glutamine 870 12 29
Thr2 to aspartic acid 8130 1250 1110
Thr2 to glutamic acid 5730 433 468
Thr2 to lysine 1670 31 70
Thr2 to arginine 5010 12 28
Thr2 to leucine    
Val4 to serine >2000 6.8 196
Ser38 to alanine    
  1. MMP, matrix metalloproteinase.