Figure 1

Production of IL-12p40 and IL-12p70 by synovial fluid (SF) macrophages and peripheral blood (PB) monocytes. Production of IL-12p70 (a,c) and IL-12p40 (b,d) by SF macrophages from patients with rheumatoid arthritis and by normal PB monocytes, cultured for 72 hours in the presence of CD154 transfectants (at a ratio of 1:5 transfectants:responder cells), control J558L-cells (1:5), LPS (1 μg/ml), or in medium only. Supernatants were collected at the end of the culture period and the production of the cytokines was determined using ELISA. Individual values from each experiment (n = 5–7) are shown. Each patient or control subject is represented by the same symbol in two panels. The detection limits for the assays (8 pg/ml for IL-12 p70 and 31 pg/ml for IL-12 p40) are indicated as broken horizontal lines. LPS, lipopolysaccharide.