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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Regulation of CD154-induced interleukin-12 production in synovial fluid macrophages

Figure 3

Production of IL-12p40 and p70 by synovial fluid (SF) macrophages precultured with IL-4 and IL-13. SF macrophages were cultured in the presence of IL-4 (n = 9) or IL-13 (n = 6) (100 U/ml) or in medium only (n = 9) for 72 hours before addition of CD154 transfectants (at a ratio of 1:5 transfectants:SF macrophages) or control J558L cells (1:5) for a further 24 hours. Supernatants were collected at the end of the culture and the production of IL-12p70 (a) and p40 (b) was determined using ELISA. Individual values from each experiment are shown; each patient is represented by the same symbol in the two panels. Cells stimulated with control J558L did not produce IL-12p40 nor p70 (data not shown).

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