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Table 3 Effect of CD40 ligation on cytokine production by dendritic cells (DCs) derived from synovial fluid (SF) macrophages or normal peripheral blood (PB) monocytesa

From: Regulation of CD154-induced interleukin-12 production in synovial fluid macrophages

 

SF-derived DCsb

PB-derived DCsc

Cytokine

J558L

CD154

J558L

CD154

IL-12p70

<8

1,072 ± 632

<8

271 ± 52

IL-12p40

61 ± 30

24,670 ± 8,127

47 ± 12b

18,432 ± 2,344b

TNF-α

<1

1,570 ± 906

4 ± 2

1,598 ± 373

IL-10

4 ± 3

223 ± 43

8 ± 3

430 ± 144

  1. aPB monocytes and SF macrophages were cultured with IL-4 and granulocyte/macrophage-colony-stimulating factor for 7 days, and irradiated control J558L-cells (1:5) or CD154 tranfectants (1:5) were added for 24 hours. Cytokine concentrations were determined using cytokine specific ELISAs. Results are presented as mean ± SEM pg/ml. bn = 3; cn = 4 except as otherwise indicated.