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Table 1 Clinical features of patients included in the study

From: Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA

  

Reverse transcription PCR using

 
  

Universal primers

Specific primers

 

Patient

Bacteria implicated

DNase

Non-DNase

Yer

Chlam

E. coli

Camp

Clinical details

A

Yersinia

6/20

2/20

+

 

+

 

Acute ReA (see [10]); positiveve serology (agglutination 1:2500) and T cell responses

B

Enteric

10/20

7/20

  

+

 

Acute ReA following gastroenteritis; organism unknown

C

Meningococci

Negative

2/20

  

+

 

Acute postinfective arthritis; sore throat, rash, positive serology; daughter had meningococcal purpura and arthritis

D

Streptococcus

17/20

5/20

  

+

 

Chronic erosive seronegative oligoarthritis; persistent positive serology – very high ASOT (>1200 U) and anti-Dnase

E

Chlamydia

5/20

5/20

 

+

+

 

Acute sexually acquired ReA; high T-cell mediated responses to CT

F

Yersinia (?)

7/20

6/20

  

+

 

Acute (?) ReA; positive serology, B27+ and T-cell responses; no enteritis

G

Unknown

12/20

1/20

  

+

 

Chronic seronegative oligoarthritis; culture positive Campylobacter infection 2 years pre-arthritis

H

Unknown

11/20

5/20

  

+

 

Chronic seronegative oligoarthritis

I

Yersinia

8/20

9/20

  

+

 

Acute ReA following gastroenteriitis; positive serology (IgM 1:640, IgG 1:2560, IgA <1:80) and T-cell responses

J

Chlamydia

7/20

3/20

  

+

 

Acute sexually acquired ReA; high T-cell mediated responses to CT

K

Campylobacter

19/20

20/20

  

+

 

Psoriatic arthritis complicated by culture positive enteritis and flare in joint symptoms (?ReA)

L

Campylobacter

19/20

16/20

  

+

 

Acute ReA following enteric infection

M

Campylobacter

Negative

Negative

    

Acute seronegative oligoarthritis and vasculitis; positiveve serology (IgM <1:80, IgG 1:640, IgA 1:160)

N

Campylobacter

Negative

Negative

    

Sero-negative oligoarthritis; positive serology (IgM 1:640, IgG 1:640, IgA 1:160) and T-cell responses

  1. Universal (DNase and non-DNase) indicates the number of clones sequenced giving good quality sequence from PCR products obtained using universal primers R1 and R2 from each patient, with and without DNase treatment. Specific indicates results of subsequent Yersinia (Yer), Chlamydia (Chlam), Escherichia coli, and Campylobacter (Camp) specific PCR performed on the products obtained using universal primers. ASOT, antistreptolysin O titre; CT, Chlamydia trachomatis.