Figure 6

Effects of anti-integrin and antiadhesion molecule neutralizing monoclonal antibodies on IFN-γ inducible protein-10 (IP-10) secretion. (a) Synovial fluid monocytes (mono) or (b) polymorphonuclear neutriphils (PMNs) were layered onto rheumatoid arthritis fibroblast-like synoviocyte (FLS) monolayers in 48-well plates, after which monoclonal antibodies (5 μg/ml) against CD11b, CD18 or intercellular adhesion molecule (ICAM)-1 were added. After incubating for 24 hours, the supernatants were harvested and assayed using enzyme-linked immunosorbent assay. Each bar represents the mean (pg/ml) ± SEM of four independent experiments, which were performed using two different rheumatoid arthritis fibroblasts and four different rheumatoid arthritis synovial fluid leukocytes. *P < 0.05, versus the respective coculture in the absence of monoclonal antibody.