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Table 1 Effects of a transwell membrane filter on IFN-γ inducible protein-10 secretion

From: A novel mechanism for the regulation of IFN-γ inducible protein-10 expression in rheumatoid arthritis

Conditions IP-10 % Inhibition
FLS 11.0 ± 7.0 ND
Monocyte 16.6 ± 5.7 ND
FLS + monocyte 5698.1 ± 865.9 -
FLS + monocyte (FLS-sup) 16.7 ± 6.7** 99.7
FLS + monocyte (monocyte-sup) 49.0 ± 13.0** 99.1
PMN 0 ND
FLS + PMN 417.4 ± 48.5 -
FLS + PMN (FLS-sup) 9.8 ± 4.1** 97.7
FLS + PMN (PMN-sup) 26.3 ± 16.0** 93.7
  1. Synovial fluid monocytes or polymorphonuclear neutrophils (PMNs) were layered onto fibroblast-like synoviocyte (FLS) monolayers in the presence or absence of a transwell membrane (pore size 0.45 μm). After 24 hours of incubation, the supernatants were collected from the cocultures and from the FLS monolayer (FLS-sup) and leukocyte suspension (monocyte-sup or PMN-sup) sides of the transwell membrane, and assayed using enzyme-linked immunosorbent assay. Values represent the mean (pg/ml) ± SEM of three independent experiments, which were performed using two different rheumatoid arthritis fibroblasts and three different rheumatoid arthritis synovial fluid leukocytes. Percentage inhibition was calculated by subtracting the IFN-γ inducible protein-10 (IP-10) contents obtained with either FLS-sup or monocyte-sup/PMN-sup from those with cocultures and dividing by the IP-10 contents obtained with cocultures (as 100%). ** P < 0.01, versus the respective coculture. ND, not done.