Figure 1

Schematic representation of the two-stage V^Hfingerprinting assay using either genomic DNA or complementary (c)DNA astemplates. In stage I for the genomic DNA-based assay, genomic DNA wasamplified using a sense V^H family-specific FR1 primer in conjunctionwith an antisense J^H consensus primer. For the cDNA-based assay,cDNA was amplified using a sense V^H family-specific FR1 primer inconjunction with the appropriate antisense CH primer. In stage II, polymerasechain reaction (PCR) products generated from either genomic DNA or cDNA wereamplified using a nested sense V_H family-specific FR3 primer and aradiolabeled antisense nested J^H consensus primer that had beenend-labeled with γ³²-P. The radiolabeled PCR products wereelectrophoresed through a 6% denaturing acrylamide sequencing gel, and thenexposed to photographic film overnight. See text (Materials and methods) forfurther details. *Areas of coding end processing at theD-J^H and V^H-DJ^H junctions. The V^Hfingerprinting results displayed were derived with the cDNA-based assay.