Figure 3
Cytometric analysis of cell proliferation. (a) Cells are labelled with carboxyfluoresceine (carboxyfluoresceine-diacetate-succinimidylester) before antigenic challenge ex vivo (2–5 days). Cells reacting to the antigen by proliferation will divide their fluorescent label among their daughter cells on each cell division. (b) Carboxyfluoresceine is directly analyzed on viable or fixed cells, and cells can be counterstained for any other parameter.