Functional analysis of an arthritogenic synovial fibroblast

Table 1 Summary of arthritis induction by transfer of TNF-expressing SFs

Derived	Transgene	Clone	Host genotype	Incidence of arthritis
Synovium	hTNF/TAg	B2	wt	31/65 (47.6%)
Synovium	hTNF/TAg	B1	wt	5/8 (62.5%)
Synovium	hTNF/TAg	A4	wt	4/8 (50.0%)
Synovium	TAg	F6	wt	0/54
Synovium	TAg	B2	wt	0/8
Synovium	TAg	A2	wt	0/8
Synovium	hTNF/TAg + Ab^a	B2	wt	0/16
Synovium	hTNF/TAg	B2	RAG^-/^-	6/10 (60.0%)
Synovium	TAg	F6	RAG^-/^-	0/9
Lung^b	hTNF/TAg	LFs	wt	0/6
Lung^b	hTNF/TAg	LFs	RAG^-/^-	0/5

Mice were classified as arthritic upon positive confirmation by histological analysis. ^a+Ab denotes group injected with arthritogenic clone B2 and then treated with anti-hTNF antibody 2 weeks after injection. ^b 'Lung' refers to a population of hTNF-secreting lung fibroblasts derived from hTNF/TAg double transgenic mice. hTNF, human tumor necrosis factor; RAG, recombinant activating gene; TAg, large tumor antigen; wt, wild-type.

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